Details of Experiment

Regulating Pair
Introduced Variation	Knockdown (shRNA) of SULF1	Protein Info
Induced Change	Cholest-5-en-3beta-yl octadecanoate concentration: decrease (FC = 0.32 / 0.61)	Metabo Info
Summary	Introduced Variation Induced Change

Full List of Experiment(s) Validating This Regulating Pair
Details of Experiment for Validating This Pair			[1]
Method	Quantifying the change of cholest-5-en-3beta-yl (7Z,10Z,13Z,16Z,19Z-docosapentaenoate) concentration after SULF1 knockdown through LC/MS neg based metabolomics.
Disease Status	Ovarian cancer	ICD-11: 2C73
Organ/Tissue/Cell Line	OV202 cells (Human ovarian cancer cells)	Ovary
Subcellular Location	Endoplasmic reticulum; Golgi apparatus (Golgi stack); Membrane (Cell surface)
Tested Species	Species: Homo sapiens (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens
Details	It is reported that knockdown of SULF1 leads to the decrease of cholest-5-en-3beta-yl octadecanoate levels compared with control group in OV202 cells.

References
1	Erratum to: Loss of HSulf-1 promotes altered lipid metabolism in ovarian cancer. Cancer Metab. 2014 Nov 4;2:24.

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