| Regulating Pair |
| Introduced Variation |
Knockdown (shRNA) of PKM |
Protein Info
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| Induced Change |
Pyruvic acid concentration: decrease |
Metabo Info
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| Summary |
Introduced Variation
Induced Change
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| Full List of Experiment(s) Validating This Regulating Pair |
| Details of Experiment for Validating This Pair |
[1] |
| Method |
Quantifying the change of pyruvic acid concentration after PKM knockdown through liquid chromatography - electrospray ionization - mass spectrometry (LCMS) based metabolomics. |
| Disease Status |
Colon cancer |
ICD-11: 2B90 |
| Organ/Tissue/Cell Line |
HCT116 cells (Human colon carcinoma cells) |
Colon |
| Subcellular Location |
Cytoplasm; Nucleus |
| Tested Species |
Species: Homo sapiens (Click to Show/Hide the Complete Species Lineage)
Kingdom: Metazoa
Phylum: Chordata
Class: Mammalia
Order: Primates
Family: Hominidae
Genus: Homo
Species: Homo sapiens
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| Details |
It is reported that knockdown of PKM leads to the decrease of pyruvic acid levels compared with control group in HCT116 cells. |
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| References |
| 1 |
Serine is a natural ligand and allosteric activator of pyruvate kinase M2. Nature. 2012 Nov 15;491(7424):458-462.
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