Details of Experiment

Regulating Pair
       Introduced Variation Overexpression of Mpc1 Protein Info
       Induced Change Pyruvic acid concentration: increase Metabo Info
       Summary Introduced Variation         Induced Change 
Full List of Experiment(s) Validating This Regulating Pair
Details of Experiment for Validating This Pair [1]
       Method Quantifying the change of pyruvic acid concentration after Mpc1 overexpression through 24-well Seahorse microplates on a Seahorse XF24 analyzer based metabolomics.
       Disease Status Colon cancer ICD-11: 2B90
       Organ/Tissue/Cell Line HCT15 cells (Human colon carcinoma cells) Colon
HT29 cells (Human colon carcinoma cells) Colon
       Subcellular Location Mitochondrion (Mitochondrion inner membrane)
       Tested Species   Species: Mus musculus  (Click to Show/Hide the Complete Species Lineage)
Kingdom: Metazoa
Phylum: Chordata
Class: Mammalia
Order: Rodentia
Family: Muridae
Genus: Mus
Species: Mus musculus
       Details It is reported that overexpression of Mpc1 leads to the increase of pyruvic acid levels compared with control group in HCT15 cells and HT29 cells.
References
1 A role for the mitochondrial pyruvate carrier as a repressor of the Warburg effect and colon cancer cell growth. Mol Cell. 2014 Nov 6;56(3):400-13.

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