Details of Experiment

Regulating Pair
Introduced Variation	Inhibition (SB334867) of HCRTR1	Protein Info
Induced Change	Lactic acid concentration: increase	Metabo Info
Summary	Introduced Variation Induced Change

Full List of Experiment(s) Validating This Regulating Pair
Details of Experiment for Validating This Pair			[1]
Method	Quantifying the change of 2-hydroxypropanoic acid concentration after HCRTR1 inhibition through kit detection.
Disease Status	Hepatocellular carcinoma	ICD-11: 2C12
Organ/Tissue/Cell Line	HepG2 cells (Human hepatoma cells)	Liver
	Hep3B (Human hepatoma cells)	Liver
	THLE3 cells (Primary normal liver cells)	Liver
Subcellular Location	Membrane (Cell membrane)
Tested Species	Species: Homo sapiens (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens
Details	It is reported that inhibition of HCRTR1 leads to the increase of lactic acid levels compared with control group in HepG2 cells, Hep3B and THLE3 cells.

References
1	Orexin A affects HepG2 human hepatocellular carcinoma cells glucose metabolism via HIF-1-dependent and -independent mechanism. PLoS One. 2017 Sep 8;12(9):e0184213.

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