Details of Experiment

Regulating Pair
       Introduced Variation Knockdown (shRNA) of PFKFB4 Protein Info
       Induced Change Glucose concentration: decrease Metabo Info
       Summary Introduced Variation         Induced Change 
Full List of Experiment(s) Validating This Regulating Pair
Details of Experiment (1) for Validating Pair [1]
       Method Quantifying the change of D-glucose concentration after PFKFB4 knockdown through 1H-NMR spectroscopy based metabolomics.
       Disease Status Lung cancer ICD-11: 2C25
       Organ/Tissue/Cell Line H460 cells (Human non-small cell lung carcinoma cells) Lung
A549 cells (Human non-small cell lung carcinoma cells) Lung
       Subcellular Location Cytoplasm (Cytosol)
       Tested Species   Species: Homo sapiens  (Click to Show/Hide the Complete Species Lineage)
Kingdom: Metazoa
Phylum: Chordata
Class: Mammalia
Order: Primates
Family: Hominidae
Genus: Homo
Species: Homo sapiens
       Details It is reported that knockdown of PFKFB4 leads to the decrease of glucose levels compared with control group in MDA-MB-231 cells and MCF-7 cells.
Details of Experiment (2) for Validating Pair [1]
       Method Quantifying the change of D-glucose concentration after PFKFB4 knockdown through 1H-NMR spectroscopy based metabolomics.
       Disease Status Colon cancer ICD-11: 2B90
       Organ/Tissue/Cell Line HCT116 cells (Human colon carcinoma cells) Colon
       Subcellular Location Cytoplasm (Cytosol)
       Tested Species   Species: Homo sapiens  (Click to Show/Hide the Complete Species Lineage)
Kingdom: Metazoa
Phylum: Chordata
Class: Mammalia
Order: Primates
Family: Hominidae
Genus: Homo
Species: Homo sapiens
       Details It is reported that knockdown of PFKFB4 leads to the decrease of glucose levels compared with control group in MDA-MB-231 cells and MCF-7 cells.
Details of Experiment (3) for Validating Pair [1]
       Method Quantifying the change of D-glucose concentration after PFKFB4 knockdown through 1H-NMR spectroscopy based metabolomics.
       Disease Status Prostate cancer ICD-11: 2C82
       Organ/Tissue/Cell Line LNCaP cells (L ymph N ode Ca rcinoma of the P rostate cells) Prostate
       Subcellular Location Cytoplasm (Cytosol)
       Tested Species   Species: Homo sapiens  (Click to Show/Hide the Complete Species Lineage)
Kingdom: Metazoa
Phylum: Chordata
Class: Mammalia
Order: Primates
Family: Hominidae
Genus: Homo
Species: Homo sapiens
       Details It is reported that knockdown of PFKFB4 leads to the decrease of glucose levels compared with control group in MDA-MB-231 cells and MCF-7 cells.
Details of Experiment (4) for Validating Pair [2], [1]
       Method Quantifying the change of D-glucose concentration after PFKFB4 knockdown through 1H-NMR spectroscopy based metabolomics.
       Disease Status Breast cancer ICD-11: 2C60
       Organ/Tissue/Cell Line MDA-MB-231 cells (Human breast cancer cells) Breast
MCF-7 cells (Human breast cancer cells) Breast
       Subcellular Location Cytoplasm (Cytosol)
       Tested Species   Species: Homo sapiens  (Click to Show/Hide the Complete Species Lineage)
Kingdom: Metazoa
Phylum: Chordata
Class: Mammalia
Order: Primates
Family: Hominidae
Genus: Homo
Species: Homo sapiens
       Details It is reported that knockdown of PFKFB4 leads to the decrease of glucose levels compared with control group in MDA-MB-231 cells and MCF-7 cells.
References
1 Fructose-2,6-bisphosphate synthesis by 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 (PFKFB4) is required for the glycolytic response to hypoxia and tumor growth. Oncotarget. 2014 Aug 30;5(16):6670-86.
2 Snail reprograms glucose metabolism by repressing phosphofructokinase PFKP allowing cancer cell survival under metabolic stress. Nat Commun. 2017 Feb 8;8:14374.

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