Details of Experiment

Regulating Pair
Introduced Variation	Knockout of Amacr	Protein Info
Induced Change	Cholesterol concentration: increase	Metabo Info
Summary	Introduced Variation Induced Change

Full List of Experiment(s) Validating This Regulating Pair
Details of Experiment (1) for Validating Pair			[1]
Method	Quantifying the change of cholesterol concentration after Amacr knockout through HILICESIMS/MS based metabolomics.
Disease Status	Lysosomal storage diseases	ICD-11: 5C56
Organ/Tissue/Cell Line	N.A.	Brain
Subcellular Location	Peroxisome; Mitochondrion
Tested Species	Species: Mus musculus (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Rodentia Family: Muridae Genus: Mus Species: Mus musculus
Details	It is reported that knockout of Amacr leads to the increase of cholesterol levels compared with control group.
Details of Experiment (2) for Validating Pair			[2]
Method	Quantifying the change of cholesterol concentration after Amacr knockout through HILICESIMS/MS based metabolomics.
Disease Status	Healthy individual
Organ/Tissue/Cell Line	N.A.	Serum
Subcellular Location	Peroxisome; Mitochondrion
Tested Species	Species: Mus musculus (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Rodentia Family: Muridae Genus: Mus Species: Mus musculus
Details	It is reported that knockout of Amacr leads to the increase of cholesterol levels compared with control group.

References
1	Role of -glucosidase 2 in aberrant glycosphingolipid metabolism: model of glucocerebrosidase deficiency in zebrafish. J Lipid Res. 2019 Nov;60(11):1851-1867.
2	Phytol is lethal for Amacr-deficient mice. Biochim Biophys Acta. 2015 Oct;1851(10):1394-405.

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