Details of Experiment

Regulating Pair
Introduced Variation	Knockout of Nat1	Protein Info
Induced Change	Isoniazid concentration: increase (FC = 1.86)	Metabo Info
Summary	Introduced Variation Induced Change

Full List of Experiment(s) Validating This Regulating Pair
Details of Experiment (1) for Validating Pair			[1]
Method	Quantifying the change of isoniazid concentration after NAT knockout through Ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-TOFMS) based metabolomics.
Disease Status	Metabolic liver disease	ICD-11: 5C90
Organ/Tissue/Cell Line	N.A.	Liver
Subcellular Location	Cytoplasm
Tested Species	Species: Mus musculus (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Rodentia Family: Muridae Genus: Mus Species: Mus musculus
Details	It is reported that knockout of NAT leads to the increase of isoniazid levels compared with control group.
Details of Experiment (2) for Validating Pair			[1]
Method	Quantifying the change of isoniazid concentration after NAT knockout through Ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-TOFMS) based metabolomics.
Disease Status	Healthy individual
Organ/Tissue/Cell Line	N.A.	Liver
Subcellular Location	Cytoplasm
Tested Species	Species: Mus musculus (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Rodentia Family: Muridae Genus: Mus Species: Mus musculus
Details	It is reported that knockout of NAT leads to the increase of isoniazid levels compared with control group.

References
1	Deficiency of N-acetyltransferase increases the interactions of isoniazid with endobiotics in mouse liver. Biochem Pharmacol. 2017 Dec 1;145:218-225.

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