Details of Experiment

Regulating Pair
Introduced Variation	(2E,4E)-9-Hydroxyoctadeca-2,4-dienoic acid addition (0.17 hours)	Metabo Info
Induced Change	MAPK8 protein phosphorylation levels: increase	Protein Info
Summary	Introduced Variation Induced Change

Full List of Experiment(s) Validating This Regulating Pair
Details of Experiment for Validating This Pair			[1]
Method	Quantifying the variation of MAPK8 protein phosphorylation abundances after 9-hydroxyoctadecadienoic acid addition through western blot analysis.
Disease Status	Atherosclerosis	ICD-11: BD40
Organ/Tissue/Cell Line	CHO-g2a cells (Chinese hamster ovary cells expressing G2A)	Ovary
Subcellular Location	Cytoplasm; Nucleus; Cell junction (Synapse)
Tested Species	Species: Homo sapiens (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens
Details	It is reported that (2E,4E)-9-hydroxyoctadeca-2,4-dienoic acid addition causes the increase of MAPK8 protein phosphorylation compared with control group in the CHO-g2a cells.

References
1	Identification of 9-hydroxyoctadecadienoic acid and other oxidized free fatty acids as ligands of the G protein-coupled receptor G2A. J Biol Chem. 2005 Dec 9;280(49):40676-83.

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