Details of Experiment

Regulating Pair
Introduced Variation	Leucine addition (288 hours)	Metabo Info
Induced Change	INS1 protein expression levels: increase	Protein Info
Summary	Introduced Variation Induced Change

Full List of Experiment(s) Validating This Regulating Pair
Details of Experiment (1) for Validating Pair			[1]
Method	Quantifying the variation of INS1 protein abundances after leucine addition through ELISA analysis or RIA analysis.
Disease Status	Healthy individual
Organ/Tissue/Cell Line	N.A.	Blood
Subcellular Location	Secreted
Tested Species	Species: Rattus norvegicus (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Rodentia Family: Muridae Genus: Rattus Species: Rattus norvegicus
Details	It is reported that leucine addition causes the increase of INS1 protein expression compared with control group in the sprague-dawley rats.
Details of Experiment (2) for Validating Pair			[2]
Method	Quantifying the variation of INS1 protein abundances after leucine addition through ELISA analysis or RIA analysis.
Disease Status	Type 2 diabetes mellitus	ICD-11: 5A11
Organ/Tissue/Cell Line	INS-1E cells (Insulin immunoreactive cells)	Pancreas islet
Subcellular Location	Secreted
Tested Species	Species: Rattus norvegicus (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Rodentia Family: Muridae Genus: Rattus Species: Rattus norvegicus
Details	It is reported that leucine addition causes the increase of INS1 protein expression compared with control group in the INS-1E cells.

References
1	Leucine in food mediates some of the postprandial rise in plasma leptin concentrations. Am J Physiol Endocrinol Metab. 2006 Sep;291(3):E621-30.
2	Chronic exposure to leucine in vitro induces -cell dysfunction in INS-1E cells and mouse islets. J Endocrinol. 2012 Oct;215(1):79-88.

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