Details of Experiment

Regulating Pair
Introduced Variation	Overexpression of KRAS	Protein Info
Induced Change	PI(18:0/20:4(5Z,8Z,11Z,14Z)) concentration: decrease (FC = 0.34)	Metabo Info
Summary	Introduced Variation Induced Change

Full List of Experiment(s) Validating This Regulating Pair
Details of Experiment for Validating This Pair			[1]
Method	Quantifying the change of PI(18:0/20:4(5Z,8Z,11Z,14Z)) concentration after KRAS overexpression through LC-MS based metabolomics.
Disease Status	Lung cancer	ICD-11: 2C25
Organ/Tissue/Cell Line	NCI-H1299 cells (Human non-small lung cancer cells)	Lung
Subcellular Location	Membrane (Cell membrane); Cytoplasm (Cytoplasmic side; Cytosol)
Tested Species	Species: Homo sapiens (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens
Details	It is reported that overexpression of KRAS leads to the decrease of PI(18:0/20:4(5Z,8Z,11Z,14Z)) levels compared with control group in NCI-H1299 cells.

References
1	Capturing the metabolomic diversity of KRAS mutants in non-small-cell lung cancer cells. Oncotarget. 2014 Jul 15;5(13):4722-31.

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