Details of Experiment

Regulating Pair
Introduced Variation	Cholesterol addition (72 hours)	Metabo Info
Induced Change	SREBF1 mRNA levels: decrease (FC = 0.2)	Protein Info
Summary	Introduced Variation Induced Change

Full List of Experiment(s) Validating This Regulating Pair
Details of Experiment for Validating This Pair			[1]
Method	Quantifying the variation of SREBF1 mRNA levels abundances after cholesterol addition through quantitative real-time RT-PCR analysis.
Disease Status	Lipid metabolism disorders	ICD-11: 5C52
Organ/Tissue/Cell Line	CYP27-deficient human fibroblasts	Skin
Subcellular Location	Endoplasmic reticulum (Endoplasmic reticulum membrane); Golgi apparatus (Golgi apparatus membrane); Cytoplasm (COPII-coated vesicle membrane)
Tested Species	Species: Homo sapiens (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens
Details	It is reported that cholesterol addition causes the decrease of SREBF1 mRNA levels compared with control group in the CYP27-deficient human fibroblasts.

References
1	27-hydroxycholesterol is an endogenous ligand for liver X receptor in cholesterol-loaded cells. J Biol Chem. 2001 Oct 19;276(42):38378-87.

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