Details of Experiment

Regulating Pair
Introduced Variation	Leucine decrease (168 hours)	Metabo Info
Induced Change	AKT1 protein phosphorylation levels: increase	Protein Info
Summary	Introduced Variation Induced Change

Full List of Experiment(s) Validating This Regulating Pair
Details of Experiment for Validating This Pair			[1]
Method	Quantifying the variation of AKT1 protein phosphorylation abundances after leucine decrease through western blot analysis.
Disease Status	Hepatocellular carcinoma	ICD-11: 2C12
Organ/Tissue/Cell Line	Primary cultured mice hepatocytes	Liver
	HepG2 cells (Human hepatocellular carcinoma cells)	Liver
	N.A.	White adipose tissue
	N.A.	Muscle
Subcellular Location	Cytoplasm; Nucleus; Membrane (Cell membrane)
Tested Species	Species: Mus musculus (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Rodentia Family: Muridae Genus: Mus Species: Mus musculus
Details	It is reported that leucine decrease causes the increase of AKT1 protein phosphorylation compared with control group in the C57BL/6J mice liver.

References
1	Leucine deprivation increases hepatic insulin sensitivity via GCN2/mTOR/S6K1 and AMPK pathways. Diabetes. 2011 Mar;60(3):746-56.

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