Details of Experiment

Regulating Pair
Introduced Variation	Methionine sulfoximine addition (6 hours)	Metabo Info
Induced Change	EIF2S1 protein phosphorylation levels: increase (FC = p-eiF2)	Protein Info
Summary	Introduced Variation Induced Change

Full List of Experiment(s) Validating This Regulating Pair
Details of Experiment for Validating This Pair			[1]
Method	Quantifying the variation of EIF2S1 protein phosphorylation abundances after methionine sulfoximine addition through western blot analysis.
Disease Status	Inflammatory bowel disease	ICD-11: DD72
Organ/Tissue/Cell Line	HCT-8 cells (Human colon tumor cells)	Intestinal epithelium
Subcellular Location	Cytoplasm (Stress granule)
Tested Species	Species: Homo sapiens (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens
Details	It is reported that methionine sulfoximine addition causes the increase of EIF2S1 protein phosphorylation compared with control group in the HCT-8 cells.

References
1	Effects of essential amino acids or glutamine deprivation on intestinal permeability and protein synthesis in HCT-8 cells: involvement of GCN2 and mTOR pathways. Amino Acids. 2012 Jan;42(1):375-83.

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